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Nay Chi Oo was born on 25 January 1992 in Yangon, Myanmar into a military family. She is the eldest daughter of Myo Myint Sein, a former military official, and his wife Than Than Swe, a botanist. Nay Chi Oo has a younger sister, Sein Lae Yadanar, and a younger brother.[6] She attended high school at Practising School Yangon Institute of Education and graduated with GCE Advanced Level from Cambridge Tutors College in 2013, and MSc Forensic Medical Sciences from University of Bradford. While still in university, she has served as Student president of Cambridge Tutors College. She studied MBA at Global MBA University of the Thai Chamber of Commerce.